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Sci Total Environ：NMT驗(yàn)證鋁毒削弱景天鎘?積累能力
NMT作為生命科學(xué)底層核心技術(shù)，是建立活體創(chuàng)新科研平臺(tái)的*技術(shù)。2005年~2020年，NMT已扎根中國15年。2020年，中國NMT銷往瑞士蘇黎世大學(xué)，正式打開歐洲市場(chǎng)。
研究使用平臺(tái)：NMT重金屬創(chuàng)新科研平臺(tái)
期刊：Science of the Total Environment
主題：NMT驗(yàn)證鋁毒削弱景天鎘積累能力
標(biāo)題：Aluminum toxicity decreases the phytoextraction capability by cadmium/zinc hyperaccumulator Sedum plumbizincicola in acid soils
影響因子：5.589
檢測(cè)指標(biāo)：Cd2+流速
檢測(cè)樣品：伴礦景天
Cd2+流實(shí)驗(yàn)處理方法：
伴礦景天幼苗在Al濃度為0、200、400μM，pH4的環(huán)境下處理2、12、24、48、72小時(shí)
Cd2+流實(shí)驗(yàn)測(cè)試液成份：
50 μM CdCl2, 0.1 mM CaCl2, MgCl2 and KCl, 1.0 mM NaCl, pH 4.00
作者：中科院南京土壤所吳龍華、李柱、周嘉文
中文摘要（谷歌機(jī)翻）
在酸性土壤中過量的鋁（Al）或在重復(fù)的植物提取過程中由于酸化而釋放的Al可能會(huì)損害超級(jí)蓄積劑的植物提取效率，但這通常被忽略。
在這里，我們*在長期（7周）和短期（72小時(shí)）以及土壤條件下，通過水培試驗(yàn)研究了Al對(duì)鎘（Cd）和鋅（Zn）超積累性景天（Sedum plumbizincicola）的毒性。鍋實(shí)驗(yàn)。在長期的水培試驗(yàn)中，即使在pH值為5.00的<100μMAl下（可溶性Al：8.74μM），也發(fā)現(xiàn)Al的可觀察到的毒性作用，與無Al處理相比，它能降低芽Cd吸收39.3％。
非損傷微測(cè)技術(shù)顯示，在48小時(shí)后用200μMAl在pH 4.00條件下處理后，根Cd2 +的流入被顯著抑制。盆栽實(shí)驗(yàn)證實(shí)，Al毒性可抑制酸性土壤中植物的生長和金屬吸收，可交換的Al為0.33 cmolc kg-1。Al脅迫引起的根部表面Cd吸附的減少可能是導(dǎo)致枝條Cd吸收減少的重要因素。
對(duì)金屬化學(xué)形態(tài)的分析表明，鋁的添加顯著影響莖中Cd和Zn的化學(xué)形態(tài)，使它們的活動(dòng)性降低，從而限制了Cd和Zn的轉(zhuǎn)運(yùn)。
在酸性土壤中和在反復(fù)的植物提取過程中，土壤中可能發(fā)生的鋁毒害是限制使用超級(jí)蓄積劑從污染土壤中去除金屬的效率的主要因素。
Net fluxes of Cd2+ at the root surface S. plumbizincicola under Al exposure in the short term. The error bars are SE, n = 8. Different letters indicate significant differences under different Al treatments at the same exposure time at P < 0.05.
英文摘要
Excessive aluminum (Al) in acid soils or Al released due to acidification during repeated phytoextraction might impair the phytoextraction efficiency of hyperaccumulators but this is often neglected.
Here, we investigate for the first time the toxicity of Al to the cadmium (Cd) and zinc (Zn) hyperaccumulator Sedum plumbizincicola with hydroponics experiments both in the long (7 weeks) and short terms (72 hours), and in soil conditions in a pot experiment. In the long-term hydroponics experiment, observable toxic effects of Al were found even at < 100 μM Al at pH 5.00 (soluble Al: 8.74 μM) which lowered shoot Cd uptake by 39.3% compared with the Al-free treatment.
Non-invasive Micro-test Technology shows that root Cd2+ influx was significantly inhibited after treatment with 200 μM Al at pH 4.00 after 48 hours. The pot experiment confirms that Al toxicity induced inhibition of plant growth and metal uptake in the acid soil with an exchangeable Al of 0.33 cmolc kg−1. Decreasing Cd adsorption at root surfaces induced by Al stress may be an important factor in declining shoot Cd uptake.
Analysis of the chemical forms of metals shows that Al addition significantly influenced the chemical forms of Cd and Zn in stems, made them restrained Cd and Zn translocation.
Aluminum toxicity that potentially occurs in acid soils and in soils during repeated phytoextraction would be a primary factor limiting metal removal efficiency from contaminated soils using hyperaccumulators.