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NMT作為生命科學(xué)底層核心技術(shù)，是建立活體創(chuàng)新科研平臺的*技術(shù)。2005年~2020年，NMT已扎根中國15年。2020年，中國NMT銷往瑞士蘇黎世大學(xué)，正式打開歐洲市場。

NMT歷*的今天

2017年06月13日，中國林科院亞熱帶林業(yè)研究所卓仁英用NMT發(fā)表了標(biāo)題為Overexpressing the Sedum alfredii CuZn Superoxide Dismutase Increased Resistance to Oxidative Stress in Transgenic Arabidopsis的研究成果。

期刊：Frontiers in Plant Science

主題：在轉(zhuǎn)基因擬南芥中過表達(dá)景天苜蓿Cu/Zn超氧化物歧化酶提高了對氧化應(yīng)激的抗性

標(biāo)題：Overexpressing the Sedum alfredii CuZn Superoxide Dismutase Increased Resistance to Oxidative Stress in Transgenic Arabidopsis

檢測指標(biāo)：Cd²⁺流速

作者：中國林科院亞熱帶林業(yè)研究所卓仁英、韓小嬌、李真

英文摘要

Superoxide dismutase (SOD) is a very important reactive oxygen species (ROS)- scavenging enzyme. In this study, the functions of a Cu/Zn SOD gene (SaCu/Zn SOD), from Sedum alfredii, a cadmium (Cd)/zinc/lead co-hyperaccumulator of the Crassulaceae, was characterized.

The expression of SaCu/Zn SOD was induced by Cd stress. Compared with wild-type (WT) plants, overexpression of SaCu/Zn SOD gene in transgenic Arabidopsis plants enhanced the antioxidative defense capacity, including SOD and peroxidase activities.

Additionally, it reduced the damage associated with the overproduction of hydrogen peroxide (H₂O₂) and superoxide radicals (O₂ ). The influence of Cd stress on ion flux across the root surface showed that overexpressing SaCu/Zn SOD in transgenic Arabidopsis plants has greater Cd uptake capacity existed in roots. A co-expression network based on microarray data showed possible oxidative regulation in Arabidopsis after Cd-induced oxidative stress, suggesting that SaCu/Zn SOD may participate in this network and enhance ROS-scavenging capability under Cd stress.

Taken together, these results suggest that overexpressing SaCu/Zn SOD increased oxidative stress resistance in transgenic Arabidopsis and provide useful information for understanding the role of SaCu/Zn SOD in response to abiotic stress.

中文摘要（谷歌機(jī)翻）

超氧化物歧化酶（SOD）是一種非常重要的活性氧（ROS）清除酶。在這項(xiàng)研究中，表征了景天科植物景天科植物鎘（Cd）/鋅/鉛超積累的Cu / Zn SOD基因（SaCu / Zn SOD）的功能。

鎘脅迫誘導(dǎo)了SaCu / Zn SOD的表達(dá)。與野生型植物相比，轉(zhuǎn)基因擬南芥植物中SaCu / Zn SOD基因的過表達(dá)增強(qiáng)了其抗氧化防御能力，包括SOD和過氧化物酶活性。

此外，它還減少了與過氧化氫（H₂O₂）和超氧自由基（O₂）過量產(chǎn)生相關(guān)的損害。Cd脅迫對根表面離子通量的影響表明，過表達(dá)SaCu / Zn SOD在轉(zhuǎn)基因擬南芥植物中具有更大的Cd吸收能力。基于微陣列數(shù)據(jù)的共表達(dá)網(wǎng)絡(luò)表明，Cd誘導(dǎo)的氧化脅迫后擬南芥中可能存在氧化調(diào)控，這表明SaCu / Zn SOD可能參與該網(wǎng)絡(luò)并增強(qiáng)Cd脅迫下的ROS清除能力。

綜上所述，這些結(jié)果表明，過表達(dá)SaCu / Zn SOD在轉(zhuǎn)基因擬南芥中提高了抗氧化脅迫的能力，并為理解SaCu / Zn SOD在響應(yīng)非生物脅迫中的作用提供了有用的信息。

此外，暴露于Cd下的細(xì)胞壁厚度下降導(dǎo)致HE細(xì)胞壁中較少的細(xì)胞壁鍵合Cd。因此，Cd誘導(dǎo)的根部特征以及根尖的改變導(dǎo)致了兩種生態(tài)型苜蓿鏈球菌對Cd吸收和積累的差異。

FIGURE 4 | Net Cd2C fluxes. Net Cd2C fluxes in the roots of transgenic (OE2, OE3, and OE4) and WT plants treated without (A) or with CdCl₂ stress (B), respectively. The average 300 s net Cd2C fluxes (C) are illustrated to highlight the trend differences. Bars indicate means  SD. Asterisks indicate significant differences at p < 0.05 and p < 0.01.