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NUCLEOSIL® C18液相色譜柱

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更新時間：2017-12-28 14:56:00瀏覽次數(shù)：4258

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NUCLEOSIL® C18液相色譜柱
※球形硅膠，多種孔徑大小，適合各類樣品※多種長度，內(nèi)徑規(guī)格，適合研究和質(zhì)檢不同要求

詳細介紹

Nucleosil C18 ※球形硅膠，多種孔徑大小，適合各類樣品※多種長度，內(nèi)徑規(guī)格，適合研究和質(zhì)檢不同要求
 

NUCLEOSIL® standard C18 phases

 

 

Octadecyl modified silica phases for HPLC

endcapped · USP L1

 

Features

Nonpolar, endcapped phases for RP chromatography
Available pore sizes 50, 100, 120, 300, 500, 1000 and 4000 Å
Available particle sizes 3 µm, 5 µm, 7 µm and 10 µm
pH stability at 20 °C: 2–8, carbon content depending on pore size (see table below)
Separation mechanism based on hydrophobic (van der Waals) interactions, slight residual silanol interactions
Corresponding NUCLEODUR® phases: see NUCLEODUR® C18 ec

For numerous applications on NUCLEOSIL® C18 phases click here:

Standard NUCLEOSIL® C18 phases are available as:
Packed EC columns for analytical HPLC
Packed VP columns for preparative HPLC
Bulk packings

 

產(chǎn)品應(yīng)用:

羊血中albendazole阿苯達唑代謝物的檢測色譜柱：Nucleosil 100-5 C18 250×4.6mm流動相：乙腈：0.01 M 磷酸=25:75  加入20mM辛烷磺酸鈉，2.5mM硫酸四丁基銨流  速：1.0毫升/分鐘進樣量：20ul溫  度：40℃檢測器：fluorescence 290 and 320nm樣  品： 1.albendazole sulphaxide       
        2.albendazole sulphone
        3.slbendazole 2-aminosulphone作  者：Batzias,G.C.;Delis,G.A.
來  源：Journal of Chromatography B,805（2004）267-274單核苷的檢測（HPLC 111270）
色譜柱：Nucleosil 100-5 C18，㏄ 250×4mm流動相：A)0.1M磷酸二氫鈉，4Mm 磷酸二氫四丁基胺，PH=6.0  B)50% A+50% 甲醇
              梯度洗脫：0-6 min.15% B.6-16 min.from 15% to 90% B.
                           16-17 min.90% B.17-17.5 min.to 15% B.
                           17.5-31.5 min.15% B.32 min流 速：0.5毫升/分鐘
樣 品:1.Hypoxanthine 2.UMP(Uridine monophosphale)
3.IMP(Inosine monophosphate)4.Inosine
5.AMP(Adenosine monophosphate)
6.ADP(Adenosine diphnosine)7.ATP(Adenosine triphosphate)
OPA衍生快速分離氨基酸（HPLC 107800）

色譜柱：Nucleosil 100-5 C18,250×4.6mm ID流動相：A:0.1M 乙酸鈉 PH=6.5. B:甲醇    梯度洗脫 20-45% B 5分鐘.45%-75% B 20分鐘流  速：1.0毫升/分鐘溫  度：22℃檢測器：UV 330nm作  者：Burbach J.P.H.(J.Chromatogr)

訂貨信息:

EC standard columns for HPLC

 

 

Ordering information

Eluent in column acetonitrile – water

 

ID

Length

 

EC guard
columns*

CC guard
columns**

100 mm

125 mm

150 mm

250 mm

NUCLEOSIL® 50-5 C18 ec · 5 µm particles · 50 Å pores · 14.5 % C · endcapped

 

 

4.6 mm

 

 

 

720098.46

 

721473.30

721829.40

NUCLEOSIL® 100-3 C18 · 3 µm particles · 100 Å pores · 15 % C · endcapped

 

 

4 mm

 

720150.40

 

720133.40

 

721022.30

721866.40

4.6 mm

720841.46

720150.46

720949.46

720133.46

 

721022.30

721866.40

NUCLEOSIL® 100-5 C18 · 5 µm particles · 100 Å pores · 15 % C · endcapped

 

 

2 mm

 

720002.20

720120.20

720014.20

 

721074.20

721602.30

3 mm

 

720002.30

720120.30

720014.30

 

721074.30

721602.30

4 mm

720141.40

720002.40

720120.40

720014.40

 

721074.30

721602.40

4.6 mm

720141.46

720002.46

720120.46

720014.46

 

721074.30

721602.40

NUCLEOSIL® 100-7 C18 · 7 µm particles · 100 Å pores · 15 % C · endcapped

 

 

4 mm

 

 

 

720018.40

 

 

 

4.6 mm

 

720951.46

720110.46

720018.46

 

 

 

NUCLEOSIL® 100-10 C18 · 10 µm particles · 100 Å pores · 15 % C · endcapped

 

 

4 mm

 

 

 

720023.40

 

 

 

4.6 mm

 

720701.46

720140.46

720023.46

 

 

 

NUCLEOSIL® 120-3 C18 · 3 µm particles · 120 Å pores · 11 % C · endcapped

 

 

4 mm

720149.40

720040.40

 

720055.40

 

721075.30

721606.40

4.6 mm

720149.46

720040.46

720740.46

720055.46

 

721075.30

721606.40

NUCLEOSIL® 120-5 C18 · 5 µm particles · 120 Å pores · 11 % C · endcapped

 

 

4 mm

 

720051.40

 

720041.40

 

721070.30

721783.40

4.6 mm

 

720051.46

720730.46

720041.46

 

721070.30

721783.40

NUCLEOSIL® 120-7 C18 · 7 µm particles · 120 Å pores · 11 % C · endcapped

 

 

4 mm

 

 

 

720042.40

 

 

 

NUCLEOSIL® 120-10 C18 · 10 µm particles · 120 Å pores · 11 % C · endcapped

 

 

4 mm

 

 

 

720043.40

 

 

 

4.6 mm

 

 

 

720043.46

 

 

 

NUCLEOSIL® 300-5 C18 · 5 µm particles · 300 Å pores · 6.5 % C · endcapped

 

 

4 mm

 

 

 

720065.40

 

721085.30

721608.40

4.6 mm

 

 

 

720065.46

 

721085.30

721608.40

NUCLEOSIL® 500-7 C18 · 7 µm particles · 500 Å pores · 2 % C · endcapped

 

 

4.6 mm

 

 

 

720074.46

 

 

 

NUCLEOSIL® 1000-7 C18 · 7 µm particles · 1000 Å pores · ~ 1 % C · endcapped

 

 

4.6 mm

 

 

 

720077.46

 

 

 

NUCLEOSIL® 4000-7 C18 · 7 µm particles · 4000 Å pores · < 1 % C · endcapped

 

 

4.6 mm

 

 

 

720085.46

 

 

 

Columns/pack ->

1

1

1

1

 

3

3

For EC columns with

2 mm ID

3 mm ID

4 mm ID

4.6 mm ID

 

REF

* EC guard columns

EC 4/2

EC 4/3

EC 4/3

EC 4/3

use the Column Protection
System guard column holder

718966

** CC guard columns

CC 8/3

CC 8/3

CC 8/4

CC 8/4

use the guard column
adapter EC

721359

 

Custom-packed EC columns with other dimensions are available on request.

 

VarioPrep columns for preparative HPLC

Ordering information

Eluent in column acetonitrile – water

 

Length

8 mm ID

10 mm ID

16 mm ID

21 mm ID

 

 

NUCLEOSIL® 100-5 C18 · particle size 5 µm, pore size 100 Å · 15 % C · endcapped

250 mm

 

715340.100

 

715340.210

 

 

NUCLEOSIL® 100-7 C18 · particle size 7 µm, pore size 100 Å · 15 % C · endcapped

250 mm

715332.80

715332.100

715332.160

715332.210

 

 

NUCLEOSIL® 300-7 C18 · particle size 7 µm, pore size 300 Å · 6.5 % C · endcapped

250 mm

 

715806.100

 

715806.210

 

 

Guard columns with NUCLEOSIL® C18

 

8 mm ID

16 mm ID

 

 

10 mm *

715360.80

715360.160

 

 

* 10 x 8 mm ID guard column cartridges can be used for 8 and 10 mm ID VarioPrep columns and require theguard column holder 8 mm (REF 718251), 10 x 16 ID guard column cartridges can be used for 16 and 21 mm ID VarioPrep columns and require the guard column holder 16 mm (REF 718256)

Custom-packed VP columns with other dimensions are available on request.

Other NUCLEOSIL® octadecyl phases than those listed above can also be custom-packed in VarioPrep columns.

 

 

 

NUCLEOSIL® C18 bulk packings

 

Ordering information

Phase

Endcapped

Carbon content

Pore size

Particle size

Pack of 10 g

Pack of 100 g

NUCLEOSIL 50-5 C18 ec

yes

14.5 %

50 Å

5 µm

712031.10

712031.100

NUCLEOSIL 100-3 C18

yes

15 %

100 Å

3 µm

712370.10

712370.100

NUCLEOSIL 100-5 C18

yes

15 %

100 Å

5 µm

712130.10

712130.100

NUCLEOSIL 100-7 C18

yes

15 %

100 Å

7 µm

712140.10

712140.100

NUCLEOSIL 100-10 C18

yes

15 %

100 Å

10 µm

712150.10

712150.100

NUCLEOSIL 120-3 C18

yes

11 %

120 Å

3 µm

712460.10

712460.100

NUCLEOSIL 120-5 C18

yes

11 %

120 Å

5 µm

712470.10

712470.100

NUCLEOSIL 120-7 C18

yes

11 %

120 Å

7 µm

712480.10

712480.100

NUCLEOSIL 120-10 C18

yes

11 %

120 Å

10 µm

712490.10

712490.100

NUCLEOSIL 300-5 C18

yes

6.5 %

300 Å

5 µm

712520.10

712520.100

NUCLEOSIL 300-7 C18

yes

6.5 %

300 Å

7 µm

712530.10

712530.100

NUCLEOSIL 300-10 C18

yes

6.5 %

300 Å

10 µm

712540.10

712540.100

NUCLEOSIL 500-7 C18

yes

2 %

500 Å

7 µm

712760.10

712760.100

NUCLEOSIL 1000-7 C18

yes

~ 1 %

1000 Å

7 µm

712790.10

712790.100

NUCLEOSIL 4000-7 C18

yes

< 1 %

4000 Å

7 µm

712926.10

712926.100

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