NUCLEODUR® C18 Isis

High purity silica phase for HPLC

Nonpolar crosslinked polymeric octadecyl modification
high steric selectivity · endcapped · 20 % C · USP L1

Properties

Key features:
Exceptional steric selectivity

Outstanding surface deactivation

Suitable for LC/MS and HPLC at pH extremes (pH 1–10)

Technical characteristics:
C18 phase with special polymeric, crosslinked surface modification
pore size 110 Å; particle sizes 1.8 μm, 3 μm and 5 μm;
carbon content 20 %

Recommended application:
Steroids, (o,p,m-) substituted aromatics, fat-soluble vitamins
For applications click here.

鰻魚中九種磺胺殘留檢測(cè)

色譜柱：EC 250×4.6mm NUCLEODUR C18 ISIS,5um
流動(dòng)相：乙腈：0.3% 醋酸水溶液     梯度洗脫
流  速：0.8毫升/分鐘
進(jìn)樣量：20 ul
溫  度：35℃
檢測(cè)器：UV 270nm
混標(biāo)濃度：1.0 ug/mL
樣  品：1.SG—磺胺胍  2.SD—磺胺嘧啶  3.SM2—磺胺二甲基嘧啶  4.SMM—磺胺甲氧嗪  5.SCP—磺胺氧噠嗪  7.SMP—磺胺甲噁唑  8.SMZ—磺胺間甲氧嘧啶  9.SDM—磺胺二甲氧嘧啶  10.SQ—磺胺喹噁啉

脂溶性維生素

 

色譜柱：NUCLEODUR C18 ISIS,5um 125×2mm
流動(dòng)相：乙腈：水=100:5
流  速：0.2毫升/分鐘
進(jìn)樣量：5 ul
柱  溫：25℃
檢測(cè)器：UV 275nm
樣  品：1.維生素A  2.維生素A乙酯  3.維生素K2  4.維生素D2  5.維生素D3    6.r-生育酚  7.a-生育酚  8.a-生育酚乙酯  9.維生素K1

藥物分析

 

色譜柱：NUCLEODUR C18 ISIS,5um 250×4.6mm
流動(dòng)相：乙腈：水=30:70
流  速：1.0毫升/分鐘
進(jìn)樣量：5 ul
溫  度：25℃
檢測(cè)器：UV 260nm
樣  品：1.地塞米松  2.倍他米松

胺類樣品的檢測(cè)

 

色譜柱：EC 150×4.6mm NUCLEODUR C18 ISIS,5um
流動(dòng)相：乙腈：50mM磷酸二氫鉀（PH=8）=40:6
流  速：1.0毫升/分鐘
進(jìn)樣量：8 ul
溫  度：25℃
檢測(cè)器：UV 254nm樣  品：1.尿嘧啶  2.吡啶  3.脫乙基阿特拉津  4.4-乙?；拎?/span>  5.4-乙基苯胺

 6.N.N-二jiajibenan  7.4-氨基蒽醍  8.3,5-二硝基-1-苯基乙基苯胺

EC standard columns for analytical HPLC

Ordering information

Eluent in column acetonitrile – water

Length ->

30 mm

50 mm

75 mm

100 mm

125 mm

150 mm

250 mm

NUCLEODUR® C18 Isis, 1.8 µm

2 mm ID

760406.20

760405.20

760396.20

760407.20

760409.20

3 mm ID

760406.30

760405.30

760407.30

4 mm ID

760406.40

760405.40

760407.40

4.6 mm ID

760406.46

760405.46

760407.46

EC guard columns*:

4 x 2 mm

761910.20

4 x 3 mm

761910.30

NUCLEODUR® C18 Isis, 3 µm

2 mm ID

760400.20

760401.20

760402.20

760403.20

760404.20

3 mm ID

760400.30

760401.30

760402.30

760403.30

760404.30

4 mm ID

760400.40

760401.40

760402.40

760403.40

760404.40

4.6 mm ID

760400.46

760397.46

760401.46

760402.46

760403.46

760404.46

EC guard columns*:

4 x 2 mm

761911.20

4 x 3 mm

761911.30

CC guard columns**:

8 x 3 mm

761300.30

8 x 4 mm

761300.40

NUCLEODUR® C18 Isis, 5 µm

2 mm ID

760410.20

760415.20

760412.20

760413.20

760414.20

3 mm ID

760410.30

760415.30

760412.30

760413.30

760414.30

4 mm ID

760410.40

760415.40

760412.40

760413.40

760414.40

4.6 mm ID

760410.46

760416.46

760415.46

760412.46

760413.46

760414.46

EC guard columns*:

4 x 2 mm

761912.20

4 x 3 mm

761912.30

CC guard columns**:

8 x 3 mm

761310.30

8 x 4 mm

761310.40

Guard columns in packs of 3, EC columns in packs of 1

Guard columns for EC columns

EC columns with ID

Use guard
column holder

2 mm

3 mm

4 mm

4.6 mm

*

Column Protection System

EC guard column

4/2

4/3

4/3

4/3

REF 718966

**

ChromCart® guard column system

CC guard column

8/3

8/3

8/4

8/4

REF 721359

Custom-packed EC columns with other dimensions are available on request.

top of page

VarioPrep columns for preparative HPLC

Ordering information

Eluent in column acetonitrile – water

Length

10 mm ID

21 mm ID

32 mm ID

40 mm ID

NUCLEODUR® C18 Isis, 5 µm

50 mm

762404.100

762404.210

125 mm

762405.100

762405.210

150 mm

762406.400

250 mm

762403.100

762403.210

762403.320

762403.400

VP guard columns with NUCLEODUR® C18 Isis

8 mm ID

16 mm ID

32 mm ID

10 mm*

762420.80

762420.160

15 mm*

762422.320

* VP guard column

Size

Pack of

for VP column

required holder

REF

VP 10/8

10 x 8 mm ID

2

10 mm ID

8 mm

718251

VP 10/16

10 x 16 mm ID

2

21 mm ID

16 mm

718256

VP 15/32

15 x 32 mm ID

1

32–40 mm ID

32 mm

718253

Custom-packed VP columns with other dimensions are available on request.

For details of the VarioPrep column system click here.

Standard program* of columns packed with NUCLEODUR® C18 Isis

Column system

Particle size:

1.8 µm

3 µm

5 µm

Analytical

EC columns (standard system, 2–4.6 mm)

Preparative

VarioPrep columns (VP, ≥ 8 mm)

 if you do not know our HPLC column systems, click the "i" for further information

* Please note! Most combinations of MN column types, available column dimensions and particle sizes can be custom-packed on request.