TwistAmp™ Basic RT TwistAmp™ Basic RT
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- 公司名稱 暢宇云商(北京)科技有限公司
- 品牌
- 型號 TwistAmp™ Basic RT
- 產地 英國
- 廠商性質 經銷商
- 更新時間 2016/5/13 16:36:41
- 訪問次數(shù) 251
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產品描述:
TwistAmp® 核酸擴增產品,能夠在15分鐘內進行常溫下的單分子核酸檢測該技術對硬件設備的要求很低,特別適合用于體外診斷、獸醫(yī)、食品安全、生物安全、農業(yè)等領域。TwistAmp Basic用于逆轉錄反應,CDNA快速擴增. 除基本組分外, 還加有通用的逆轉錄酶,但不含Rnase抑制劑。
Basic Information
RPA
1) Primers must be 30-35 bases
2) Works best at constant temperature (40-42°C)
3) Amplicons of 80-400bp are
Set-up (single-plex)1
1) Prepare reaction mix in 1.5ml tube:
Primer A (10μM) 2.4 μl
Primer B (10μM) 2.4 μl
Rehydration Buffer 29.5 μl
Template,
RNase Inhibitor and dH2O 13.2 μl
(Total Volume 47.5 μl)
Vortex and spin briefly
2) Add reaction mix to freeze-dried reaction. Pipette to mix.
3) Add 2.5 μl of 280mM MgAc (supplied) and mix well to start reaction.
4) Incubate at 40-42°C for 20-40 minutes.
4b) For low template copy number,
remove strip after 5-7 minutes, invert
vigorously 8-10 times to mix & spin
briefly, replace in heating device.
5) After 20-40 minutes, clean amplicons before running on agarose gels.
WARNING: RPA REACTIONS START AT ROOM TEMPERATURE AS SOON AS
MAGNESIUM IS ADDED.