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銅離子轉(zhuǎn)運(yùn)蛋白1抗體說(shuō)明書(shū)
閱讀：469 發(fā)布時(shí)間：2018-7-24產(chǎn)品編號(hào)bs-10773R 英文名稱(chēng)CTR1
中文名稱(chēng)銅離子轉(zhuǎn)運(yùn)蛋白1抗體
別 名Copper transport 1 homolog; Copper transporter 1; COPT1; COPT1_HUMAN; CTR1; hCTR1; High affinity copper uptake protein 1; SLC31A1; solute carrier family 31 (copper tansporters) member 1; Solute carrier family 31 member 1. 規(guī)格價(jià)格100ul 200ul
研究領(lǐng)域神經(jīng)生物學(xué) 通道蛋白 細(xì)胞膜受體抗體來(lái)源Rabbit
克隆類(lèi)型Polyclonal
交叉反應(yīng) Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit, Sheep, 產(chǎn)品應(yīng)用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800
ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user. 分 子 量21kDa
細(xì)胞定位細(xì)胞膜
性 狀Lyophilized or Liquid 濃 度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human CTR1:51-150/190
<Cytoplasmic> 亞 型IgG
純化方法affinity purified by Protein A
儲(chǔ) 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH
7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
產(chǎn)品介紹background:
The protein encoded by this gene is a high-affinity copper transporter found in the cell membrane. The encoded protein functions as a homotrimer to effect the uptake of dietary copper. [provided by RefSeq, Aug 2011]
Function:
High-affinity, saturable copper transporter involved in dietary copper uptake.
Subunit:
Homotrimer.
Subcellular Location:
Cell membrane. Localizes to the apical membrane in intestinal epithelial cells.
Post-translational modifications:
O-Glycosylation at Thr-27 protects from proteolytic cleavage in the N-terminal extracellular domain.
Similarity:
Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.
SWISS:
O15431
Gene ID:
1317
Database links:
Entrez Gene: 1317 Human Entrez Gene: 20529 Mouse Entrez Gene: 171135 Rat Omim: 603085 Human SwissProt: O15431 Human SwissProt: Q8K211 Mouse SwissProt: Q9JK41 Rat Unigene: 532315 Human
Unigene: 248637 Mouse
Unigene: 2789 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品圖片：
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; |
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Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by | ||||
3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; |
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Incubation: Anti-CTR1 Polyclonal Antibody, Unconjugated(bs-10773R) 1:200, overnight at 4°C, followed by |
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conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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