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NMT歷*的今天

2015年10月14日，浙江理工大學(xué)熊杰、中國(guó)水稻所陶龍興利用NMT在Environmental and Experimental Botany上發(fā)表了標(biāo)題為Excessive nitrate enhances cadmium (Cd) uptake by up-regulating the expression of OsIRT1 in rice (Oryza sativa)的研究成果。

期刊：Environmental and Experimental Botany

主題：過(guò)量的硝酸鹽通過(guò)上調(diào)水稻（Oryza sativa）中OsIRT1的表達(dá)來(lái)提高鎘（Cd）的吸收

標(biāo)題：Excessive nitrate enhances cadmium (Cd) uptake by up-regulating the expression of OsIRT1 in rice (Oryza sativa)

影響因子：3.712

檢測(cè)指標(biāo)：Cd2+流速

檢測(cè)部位：水稻根部

Cd2+流實(shí)驗(yàn)處理方法：

三周齡的水稻幼苗，在不同的NO3濃度（0mM、2.86mM、5.72mM）下培養(yǎng)7天，30 mM Cd2+處理30-60min

Cd2+流實(shí)驗(yàn)測(cè)試液成份：

30 μM CdCl2, 0.1 mM KCl, 0.05 mM MgCl2, 0.5 mM NaCl, 0.1 mM Na2SO4, 0.3 mM MES, pH 5.5

作者：浙江理工大學(xué)熊杰、中國(guó)水稻所陶龍興

英文摘要

In order to pursue higher yield of rice, higher amount of NO3− is often used in rice production, but limited information is available on cadmium (Cd) uptake promoted by nitrate (NO3−) in rice.

Here, hydroponic experiments with integration of three NO3− levels (0 mM, 2.86 mM and 5.72 mM simplify as 0, NO3− and 2NO3−) and two Cd concentrations (0 and 100 μM) were conducted. Phenotypes and physiological indexes related to Cd uptake were measured; content changes of nitrogen, Cd and Fe also were measured. In addition, net Cd2+ flux was monitored and quantitative RT-PCR was applied to determine the expression of Fe/Cd transporters.

The results showed that although excessive NO3− had no significant promoting effect on rice growth in absence or presence of Cd, it enhanced Cd influx in elongation zone of rice roots and increased Cd accumulation in rice plants and grains. Excessive NO3− also increased OsIRT1 expression and Fe accumulation in rice seedlings in the absence or presence of Cd.

In conclusion, excessive NO3− increases OsIRT1 expression and Cd influx in elongation zone of rice roots, and then it increased Cd and Fe uptake and accumulation in rice plants and grains, which raises an increased-risk for human health.

                                                                  中文摘要（谷歌機(jī)翻）
為了追求更高的水稻產(chǎn)量，通常在水稻生產(chǎn)中使用大量的NO3-，但是關(guān)于水稻中硝酸鹽（NO3-）促進(jìn)鎘（Cd）吸收的信息有限。

在這里，進(jìn)行了整合三個(gè)NO3-濃度（0 mM，2.86 mM和5.72 mM簡(jiǎn)化為0，NO3-和2NO3-）和兩個(gè)Cd濃度（0和100μM）的水培實(shí)驗(yàn)。測(cè)量與鎘吸收有關(guān)的表型和生理指標(biāo)；還測(cè)量了氮，鎘和鐵的含量變化。此外，監(jiān)測(cè)凈Cd2 +通量并應(yīng)用定量RT-PCR確定Fe / Cd轉(zhuǎn)運(yùn)蛋白的表達(dá)。

結(jié)果表明，盡管過(guò)量的NO3-在沒(méi)有或沒(méi)有Cd的情況下對(duì)水稻的生長(zhǎng)沒(méi)有明顯的促進(jìn)作用，但是它增加了Cd在水稻根部伸長(zhǎng)區(qū)的流入量，并增加了Cd在水稻和谷物中的積累。在沒(méi)有或沒(méi)有鎘的情況下，過(guò)量的NO3-也會(huì)增加水稻幼苗中OsIRT1的表達(dá)和鐵的積累。

總之，過(guò)量的NO3-會(huì)增加稻根伸長(zhǎng)區(qū)OsIRT1的表達(dá)和Cd的流入，然后增加稻米和谷物中Cd和Fe的吸收和積累，從而增加人體健康的風(fēng)險(xiǎn)。

Effects of different concentrations of nitrate (NO3??) on root net Cd2+ in?ux in rice seedlings. Root net Cd2+ in?ux of 3-week-old rice seedlings c*ted in 0, 2.86 and 5.72 mM NO3?? conditions for 7 days. Then, 30 mM Cd2+ were added into the solution for 30–60 min. Net Cd ?ux of meristematic zone (B and D) and elongation zone (A and C) of the main root were monitored and steady-state Cd ?ux was recorded for 10 min. Bar indicates 50 mm in (A and B), the values are means ?? SE (n = 107), different letters on the bar indicate signi?cant differences at P = 0.05.