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FOOD CHEM南京東大鈣參與鹽脅迫下作物酚類積累的GABA信號轉(zhuǎn)導(dǎo)
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期刊：Food Chemistry
主題：鈣參與鹽脅迫下作物酚類積累的GABA信號轉(zhuǎn)導(dǎo)（鈣信號）
標(biāo)題：Ca2+ involved in GABA signal transduction for phenolics accumulation in germinated hulless barley under NaCl stress
影響因子：5.399
檢測指標(biāo)：Ca2+流速
檢測部位：根尖細(xì)胞
Ca2+流實驗處理方法：
三日齡的大麥幼苗，60mM NaCl、60mM NaCl + 0.5mM GABA、60mM NaCl + 0.1mM 3-MP分別處理四天和六天
Ca2+流實驗測試液成份：
0.1mM KCl, 0.1mM CaCl2, 0.1mM MgCl2, 0.5mM NaCl, 0.3mM MES, 0.2mM Na2SO4，ph 6.0
通訊作者：南京農(nóng)業(yè)大學(xué)楊潤強(qiáng)、馬燕
英文摘要
In this study, in order to investigate the role of Ca2+ in GABA signal transduction involved in phenolics accumulation in barley seedlings under NaCl stress, the seedlings were treated with exogenous GABA and its synthesis inhibitor, 3-mercaplopropionic acid (3-MP), as well as Ca2+ channel blockers La3+, Ca2+ chelator EGTA, and Ca2+ release channel inhibitor 2-aminoethoxydiphenyl borate (2-APB).
The results showed that GABA significantly enhanced phenolics, calcium and calmodulin content. It also induced Ca2+ influx in barley root tips cells, and altered the distribution of Ca2+, making calcium precipitates more uniform and intensive. While, 3-MP treatment led to opposite changes, which suggested that GABA was essential for calcium content increase. In addition, accumulation of phenolics was inhibited by LaCl3, EGTA and 2-APB treatments, and this inhibition could be alleviated partly by exogenous GABA.
Taken together, Ca2+ was involved in GABA signal transduction for phenolics accumulation in barley seedlings under NaCl stress.
中文摘要（谷歌機(jī)翻）
在本研究中，為了研究Ca2+在NaCl脅迫下大麥幼苗中酚類物質(zhì)積累的GABA信號轉(zhuǎn)導(dǎo)中的作用，用外源GABA及其合成抑制劑3-巰基丙酸（3-MP）處理幼苗。以及Ca2+通道阻滯劑La3+，Ca2+螯合劑EGTA和Ca2+釋放通道抑制劑2-氨基乙氧基二苯基硼酸鹽（2-APB）。
結(jié)果表明，GABA顯著增強(qiáng)了酚類，鈣和鈣調(diào)蛋白的含量。它還誘導(dǎo)大麥根尖細(xì)胞內(nèi)的Ca2+流入，并改變Ca2+的分布，使鈣沉淀更均勻和密集。然而，3-MP處理導(dǎo)致相反的變化，這表明GABA對鈣含量的增加至關(guān)重要。此外，LaCl3，EGTA和2-APB處理抑制了酚類物質(zhì)的積累，這種抑制作用可以通過外源性GABA部分緩解。
總之，Ca2+參與了NaCl脅迫下大麥幼苗中酚類物質(zhì)積累的GABA信號轉(zhuǎn)導(dǎo)。
結(jié)果表明，對照大麥幼苗根尖Ca2+外排，與對照相比，NaCl處理引起的Ca2+由外排向吸收轉(zhuǎn)變。GABA處理得到穩(wěn)定的Ca2+吸收，其中處理6天的吸收明顯高于處理4天吸收。GAD抑制劑（3-MP）在不同處理時期產(chǎn)生了不同的趨勢，與單獨使用NaCl處理相比，第4天Ca2+的外排速率顯著增加，但第6天Ca2+流入量較弱。
注：CK表示大麥用蒸餾水噴灑; N表示60mM NaCl處理; NG表示60mM NaCl + 0.5mM GABA處理; NM表示60mM NaCl + 0.1mM 3-MP處理。