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期刊：Plant Journal

主題：Plant J | 非損傷微測技術(shù)突出貢獻(xiàn)獎(jiǎng)獲得者 抗鹽成果

標(biāo)題：Root vacuolar Na+ sequestration but not exclusion from uptake correlates with barley salt tolerance

影響因子：5.775

檢測指標(biāo)：Na+、H+

通訊作者：塔斯馬尼亞大學(xué)Sergey Shabala，華中農(nóng)業(yè)大學(xué)吳洪洪

英文摘要

Soil salinity is a major constraint for the global agricultural production. For many decades, Na+ exclusion from uptake has been the key trait targeted in breeding programs; yet, no major breakthrough in creating salt tolerant germplasm was achieved.

In this work, we have combined the MIFE technique for non‐invasive ion flux measurements with confocal fluorescence dye imaging technique to screen 45 accessions of barley to reveal the relative contribution of Na+ exclusion from the cytosol to the apoplast and its vacuolar sequestration in the root apex, for the overall salinity stress tolerance.

We show that Na+/H+ antiporter‐mediated Na+ extrusion from the root plays a minor role in the overall salt tolerance in barley. At the same time, a strong and positive correlation was found between root vacuolar Na+ sequestration ability and the overall salt tolerance. The inability of salt sensitive genotypes to sequester Na+ in root vacuoles was in a contrast to a significantly higher expression levels of both HvNHX1 tonoplast Na+/H+ antiporters and HvVP1 H+‐pumps compared with tolerant genotypes. This data is interpreted as a failure of sensitive varieties to prevent Na+ back‐leak into the cytosol and existence of a futile Na+ cycle at the tonoplast.

Taken together, our results demonstrated that root vacuolar Na+ sequestration but not exclusion from uptake played the main role in barley salinity tolerance and suggested that the focus of the breeding programs should be shifted from targeting genes mediating Na+ exclusion from uptake by roots to more efficient root vacuolar Na+ sequestration.

中文摘要（谷歌機(jī)翻）

土壤鹽分是農(nóng)業(yè)生產(chǎn)的主要制約因素。幾十年來，Na+被吸收排除一直是育種計(jì)劃的關(guān)鍵特征；然而，在制備耐鹽種質(zhì)方面沒有取得重大突破。

在這項(xiàng)工作中，我們將非侵入性離子通量測量的MIFE技術(shù)與共聚焦熒光染料成像技術(shù)結(jié)合起來篩選45份大麥品種，以揭示Na+從細(xì)胞質(zhì)中排斥到質(zhì)外體的相對(duì)貢獻(xiàn)及其在根中的液泡隔離頂點(diǎn)，對(duì)于整體鹽度脅迫耐受性。

我們顯示Na+/H+逆向轉(zhuǎn)運(yùn)蛋白介導(dǎo)的Na+從根部擠出在大麥的總體耐鹽性中起著次要作用。同時(shí)，根液泡Na+螯合能力與總體耐鹽性之間存在強(qiáng)烈的正相關(guān)關(guān)系。與耐受基因型相比，鹽敏感基因型不能在根空泡中螯合Na+與HvNHX1液泡膜Na+/H+逆向轉(zhuǎn)運(yùn)蛋白和HvVP1H+-泵的顯著更高表達(dá)水平形成對(duì)比。該數(shù)據(jù)被解釋為敏感品種的失敗，以防止Na+背泄漏到胞質(zhì)溶膠中并且在液泡膜中存在無效的Na+循環(huán)。

總之，我們的結(jié)果表明根液泡Na+隔離但不排除攝取在大麥耐鹽性中起主要作用，并且表明育種計(jì)劃的重點(diǎn)應(yīng)該從靶向基因介導(dǎo)Na+排斥從根吸收到更有效的根液泡Na+隔離。

Plant Journal | 根系液泡隔離Na+的活性是大麥耐鹽的關(guān)鍵參數(shù)

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