LightCycler ® EvoScript RNA 探針大師

The LightCycler® EvoScript RNA Probes Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays.

LightCycler EvoScript RNA探針Master專為敏感、高特異性、高精度一步RT-qPCR而設(shè)計(jì)，特別適用于基因表達(dá)分析。

供一般實(shí)驗(yàn)室使用。

Product No：07800029001   產(chǎn)品編號(hào)：07800029001  

Pack Size：200 x 20 µL reactions  包裝尺寸：200 x 20 µL 反應(yīng)

Product No：07800096001 產(chǎn)品編號(hào)：07800096001  

Pack Size：1000 x 20 µL reactions   包裝尺寸：1000 x 20 µL 反應(yīng)

Keywords: Hydrolysis Probes  關(guān)鍵詞： 水解探針

概述：

The LightCycler® EvoScript RNA Probes Master (1-vial composition) is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays.

LightCycler®EvoScript RNA Probes Master（1 瓶組合物）專為靈敏、高特異性、高精度的一步法 RT-qPCR 而設(shè)計(jì)，尤其適用于基因表達(dá)分析。

Ease of use, convenience, speed, reduced error

Result consistency, stability and tolerance of targets of varying secondary structure

Ability to detect low copy or rare transcripts for gene expression applications

Superior quantification capability for gene expression applications

Easier assay optimization

Contamination control capability

Generate consistent and robust results, no matter what sample type you are starting from or what target you are detecting

Rely on a higher sensitivity when detecting low copy numbers, with the input of larger sample volumes into the reaction

Obtain fast and specific results

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• 適用簡單、方便快捷、減少錯(cuò)誤

• 結(jié)果表明，不同二級結(jié)構(gòu)的目標(biāo)具有一致性、穩(wěn)定性和耐受性

• 能夠檢測低拷貝或罕見的轉(zhuǎn)錄本的基因表達(dá)應(yīng)用

• 基因表達(dá)應(yīng)用定量能力

• 更容易分析優(yōu)化

• 污染控制能力

• 生成一致且可靠的結(jié)果，無論您從什么樣本類型開始或檢測什么指標(biāo)

• 在檢測低拷貝數(shù)時(shí)依靠更高的靈敏度，將更大的樣品體積輸入到反應(yīng)中

• 獲得快速和具體的結(jié)果

Contents

Master Mix, 5× conc. Contains enzymes for reverse transcription and PCR, RT-qPCR Reaction Buffer, dATP, dCTP, dGTP, and dUTP, Mg(OAc)2, and proprietary additive

07 800 029 001   2 vials, 440 μL each

07 800 096 001 10 vials, 440 μL each

Water, PCR grade To adjust the final reaction volume.

07 800 029 001   3 vials, 1 mL each

07 800 096 001   2 vials, 7.25 mL each

內(nèi)容

• 反應(yīng)混合物，5× conc.包含逆轉(zhuǎn)錄和PCR酶，RT-qPCR反應(yīng)緩沖酶，dATP, dCTP, dGTP, dUTP, Mg(OAc)2和專有添加劑

• 07 800 029 001   2瓶，每瓶 440 μL
  07 800 096 001   10瓶，每瓶440μL

• 水，PCR 級 調(diào)整最終反應(yīng)體積。
  07 800 029 001   3瓶，每瓶 1 mL
  07 800 096 001   2瓶，每瓶 7.25 mL

應(yīng)用：

The LightCycler® EvoScript RNA Probes Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays. The 1-vial composition is ideally suited for easy reaction assembly, requiring only the addition of primers, probe and target RNA. The proprietary reaction buffer allows a convenient hot start RT-qPCR.

LightCycler EvoScript RNA探針Master是專為敏感、高特異性、高精度一步RT-qPCR而設(shè)計(jì)的，特別適用于基因表達(dá)分析。1瓶的組合物非常適合簡單的反應(yīng)組裝，只需要添加引物、探針和目標(biāo)RNA。專有的反應(yīng)緩沖液允許方便的熱啟動(dòng)RT-qPCR。

In addition, the enzymes for reverse transcription and for PCR feature hot start for premium specificity1).

The master mix is optimized for hydrolysis probes, including Universal ProbeLibrary (UPL) probes and does not require optimization with Mg(OAc)2.

There are protocols for both the LightCycler® 480 instrument and the LightCycler® 96 instrument.

1) The reverse transcription activity will be suppressed at temperatures significantly below 60°C.

此外，用于逆轉(zhuǎn)錄和 PCR 的酶具有熱啟動(dòng)特性，具有的特異性1)。

預(yù)混液針對水解探針進(jìn)行了優(yōu)化，包括 Universal ProbeLibrary (UPL) 探針，不需要使用 Mg(OAc) 2進(jìn)行優(yōu)化。

LightCycler ®  480 儀器和 LightCycler都有協(xié)議®  96 儀器。

1) 顯著低于 60°C 的溫度會(huì)抑制逆轉(zhuǎn)錄活性。