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蛋白凝膠電泳及相關(guān)的Western-Blot是蛋白分析的主流技術(shù) (Figure 1)。Applied BioProbes 公司提供兩種蛋白染料： 1)Coomassie® Blue Fast Stain, 一種即用型的蛋白染料，無需甲醇或乙酸固定、或脫色； 2) eLuminol™ Protein Gel Stain，一種具有*靈敏度的蛋白熒光染料，用于檢測SDS-PAGE凝膠電泳中的總蛋白。Applied BioProbes 公司也提供一種專門檢測磷酸化蛋白的熒光染料： Phos-Tag™ Phosphoprotein Gel Stain。Applied BioProbes提供的 ECL Western Blotting Detection Kit用于WB檢測HRP標(biāo)記的抗體及其關(guān)聯(lián)的抗原，具有快速檢測、靈敏度*等特點(diǎn)。另外，Applied BioProbes也提供了兩種蛋白定量分析試劑盒： 1) Bradford Protein Assay Kit；和 2) BCA Protein Assay Kit。

Figure 1. Protein gel analysis workflow

蛋白染料選購指南

Figure 2. Gel stain results with Coomassie® Blue Fast Stain (left), eLuminol™ Protein Gel Stain (middle), and Phos-Tag™ Phosphoprotein Gel Stain, followed by eLuminol™ total protein stain (right). Two-fold serial dilutions (from left to right) of protein marker were separated via SDS-PAGE and then stained with above stains.

Figure 3. Detection of protein in Western Blots with ABP kit (left) and Millipore Kit (right).

Figure 4. BSA standard curve with Bradford Protein Assay Kit (left) and BCA Protein Assay Kit (right).